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KMID : 0903619940350060540
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Journal of the Korean Society for Horticultural Science
1994 Volume.35 No. 6 p.540 ~ p.546
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Effect of KOH on the Seed Structure and the Physiology of Seed Germination in chrysanthemum coronarium L .
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Abstract
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The roles of KOH treatment on the seed coat (pericarp) changes and water uptakes during the early imbibition in Chrysanthemum coronarium seeds (fruit) were investigated. ,Seeds were pretreated with 1% KOH for 30 minutes, washed for 6 hours in 25℃ water and dried.
The surface characteristics of the seedcoat were examined by using scanning electron micrographs (SEM) and light micrograph. SEM revealed the wax of cuticular surface of the seedcoat to a reduced state in KOH treated seed. Moreover the thickness of cuticular layer was shown to reduced in light microscope.
Treatment with KOH exhibited an increase in water uptake. Fresh weight of seedlings was increased as from rapid water uptake and the dry weight decreased. Perhaps it might be caused by the consumption of stored materials during the respiration process. Three different stages of water uptake during the seed germination were noticed ; first increase in water uptake, steady continuation of the uptake, and second slow increase in the uptake. The later increase in the water uptake was stimulated in KOH treatment, possibly as a result of changes of seed coat (pericarp) structure. The promotion of germination is therefore, presumed due to an increase in the water uptake as brought about by KOH treatment under these favorable conditions for the germination.
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